Journal: International Journal of Molecular Medicine
Article Title: Expression levels of hnRNP K and p21 WAF1/CIP1 are associated with resistance to radiochemotherapy independent of p53 pathway activation in rectal adenocarcinoma
doi: 10.3892/ijmm.2018.3898
Figure Lengend Snippet: Analysis of intracellular signaling pathways. (A) siRNA knockdown of hnRNP K was confirmed by western immunoblotting. (B) Increases in p53 Ser15-phosphorylation, PARP cleavage and CHK2 (Thr68) phosphorylation upon ionizing radiation in (a) Colo320 and (b) SW480 cells were neither affected by mock-siRNA nor by hnRNP K-siRNA transfection. hnRNP K, heterogeneous nuclear ribonucleoprotein K; PARP, poly (ADP-ribose) polymerase; siRNA, small interfering RNA; Con, control.
Article Snippet: The SW480 and Colo320 CRC cell lines were newly purchased from the Leibniz Institute DSMZ (Braunschweig, Germany).
Techniques: Protein-Protein interactions, Knockdown, Western Blot, Phospho-proteomics, Transfection, Small Interfering RNA, Control